The National Centre for Biomolecular Research concentrates on unravelling the intricate cellular mechanisms at the molecular scale. Our approach utilizes a diverse array of in-house methodologies and specialized expertise. Research teams form a synergistic network, delving into crucial cellular processes such as gene expression, regulation, and non-coding genome, together with the interdisciplinary field of functional genomics and proteomics. Part of the topics represents essential building blocks to understanding cellular signalling, such as glycobiochemistry or interactions of proteins with DNA or membranes. Our experimental strategies are enriched by advanced computational methods, paving the way for creating innovative tools and deepening our understanding of biomolecular interactions and reaction mechanisms. We also focus on plant biology by exploring molecular complexes using genomics and proteomics approaches.