Single-molecule analysis of CRISPR-Cas enzymes, Dr. Dina Grohman

CRISPR-Cas systems constitute the adaptive immune systems of bacteria and archaea. As we scientist only discoverd CRISPR-Cas a few years ago, there is much to be learned about the diversity and function of these complex defence systems. Employing biophysical technqiues like single-molecule FRET, we seek to understand the molecular mechanisms and conformational dynamics of a subset of Cas proteins.